FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10177739
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC455740.4477811820483901No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT419580.41225266240370284No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA305150.29982101132677896No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT254420.2499769349557893No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA252460.24805116342637595No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT148240.14565120995930433No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA123260.12110744832422995No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT116590.11455392990525695No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA108240.10634975017535821No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA107580.10570127608892309No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT105980.10412921769756525No Hit
CGGTGGATTTTTCGGTTTAAGTTTTGGTAATACGGTGAAATTTCGTTTTA102560.10076894288603785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC212800.0116.13531
CGGGTGC64750.0114.90761
CGGTTAA485100.0110.5533751
CGGGTAC79650.0106.788621
GGGCGCG242500.0106.078742
CGGCTAA4000.0105.6517941
GGCGCGG252550.098.911933
CGCGGTG253850.097.4445345
CGGGTAT235200.097.103081
GCGCGGT252950.096.662344
CGGGCGT291100.095.120921
CGGATAC9100.094.842961
CGGAATA165100.094.8172151
CGGGTGT201050.094.649231
CGGTAAT70050.093.892881
CGGGGAC15900.092.8395541
CGGGAGG707950.092.585321
GGGAGGC295450.092.423652
CGGATGC6700.092.392561
CGGGTAA58300.090.253121
Produced by FastQC (version 0.11.5)