FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6779114
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC344290.5078687273882694No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC327550.4831752349938355TruSeq Adapter, Index 6 (100% over 50bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT93530.13796788193855422No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT85860.12665371905532197No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT81400.12007468822621953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC163350.0116.506781
CGGGTGC51750.0114.729811
CGGCTAA2450.0111.698631
CGGTTAA349600.0109.436761
CGGGTAC62600.0107.959311
GGGCGCG182900.0107.39272
GGCGCGG191200.099.609733
GCGCGGT187600.099.3665854
CGCGGTG190700.099.0909045
CGGGTAT180850.096.5814361
CGGGTGT154450.095.2561
CGGGCGT228850.092.9874041
CGGGTTT312450.091.926951
CGGTAAT48250.091.610991
GCGGTGG274500.091.0844046
CGGTGGT339750.090.279317
CGGATAC6750.089.898521
CGGGTAA38850.087.43821
CGGCTAT750.087.254441
CGGAAAT37300.086.605831
Produced by FastQC (version 0.11.5)