FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005416455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8160774
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC387530.47486917294854625No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT171400.21002909773019077No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA134590.16492308205079567No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT122880.1505739529118194No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA114060.13976615453387142No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC102970.12617675725366248TruSeq Adapter, Index 6 (100% over 50bp)
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA102950.12615224977434739No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT96880.11871422980222218No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT94890.11627573561037226No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)