FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7983836
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC364640.4567228084344418No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT151120.18928244518048717No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT110020.13780343183402063No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA107130.13418361800016934No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA102590.12849712844802924No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC88710.11111200179963618TruSeq Adapter, Index 6 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT87440.10952128776192296No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT85070.10655278991201723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC174250.0115.888221
CGGGTGC55900.0113.168381
CGGTTAA422150.0112.927161
CGGGTAC66600.0112.606611
CGGCTAA3000.0107.2222441
GGGCGCG199250.0106.2555852
GGCGCGG206800.099.495863
CGCGGTG206150.098.221475
GCGCGGT204900.098.2114644
CGGGTGT173450.097.328031
CGGGTAT193900.097.016741
CGGGCGT258050.091.527531
CGGAAAT45650.091.341821
CGGATGC6000.091.337471
CGGGTTT357700.091.2421
GCGGTGG300200.091.116356
CGGAATA87750.091.099881
CGGTAAT55850.090.23181
CGGTGGT374450.089.928917
GGGAGGC201500.089.358942
Produced by FastQC (version 0.11.5)