FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13114014
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC641920.4894916232360283No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT281980.2150218842224814No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA216410.16502193760049363No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA201040.1533016511954311No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA191100.145721973455267No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT185630.14155086306908016No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT168860.12876301641892407No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT160090.1220755140264453No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC135530.10334745715537591TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC308850.0114.638191
CGGGTGC94500.0113.390131
CGGGTAC119950.0112.584091
CGGTTAA683700.0112.427921
GGGCGCG349150.0105.441922
CGGCTAA4400.0101.584151
GGCGCGG365850.097.489383
CGGGTAT329000.097.237551
CGCGGTG366000.096.863955
GCGCGGT361400.096.746294
CGGAATA155100.095.2153241
CGGGTGT284050.094.917251
CGGGCGT421100.092.231631
CGGAAAT74900.091.9799961
CGGGTTT616700.091.5152361
CGGTAAT92700.091.290591
CGGATGC10050.090.7282941
GGGAGGC334500.089.6072542
CGGGAGG833750.089.485181
CGGTGGT658650.089.333277
Produced by FastQC (version 0.11.5)