FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14209778
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC727070.5116687959516327No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT310390.2184340951702412No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA229490.16150146751061134No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA209370.14734220337573184No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT190430.13401335334021405No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT177180.12468878824144895No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT176820.12443544156706741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC344950.0115.706351
CGGGTGC106600.0113.834111
CGGGTAC132050.0112.330981
CGGTTAA706300.0112.2100141
GGGCGCG388950.0106.455292
CGGCTAA4400.0105.604031
GGCGCGG406850.098.843543
CGCGGTG405100.098.5941245
GCGCGGT403200.097.716394
CGGGTAT386500.096.84031
CGGGTGT317100.095.8291551
CGGAATA165400.092.886891
CGGGCGT504750.092.56451
CGGATGC10750.091.435331
CGGGTTT670250.091.33261
GCGGTGG584500.090.9209756
CGGTAAT99250.090.812781
CGGTGGT725600.090.438157
CGGAAAT79400.090.107531
GGGAGGC362600.089.0489042
Produced by FastQC (version 0.11.5)