FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15768710
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT835020.5295423658625213No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC695640.44115212975569973No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA625050.3963862611462828No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT442780.280796590209345No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA430360.27292023253645986No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT295990.1877071745247392No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA254570.1614399656027665No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT245160.1554724514560798No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA241450.1531196908307655No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA227190.14407646535449No Hit
CGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA207150.131367752974086No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT203940.12933207599099736No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGAT194320.1232313867145759No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT191230.12127180980562137No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA178980.113503260571093No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT176980.11223492600219041No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA175650.11139148351387018No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT170560.1081635720360131No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA170480.10811283865325698No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT167580.10627375352834822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC326200.0116.270271
CGGGTGC103200.0113.968311
CGGTTAA710000.0111.5574651
CGGGTAC119600.0110.135431
GGGCGCG367550.0107.180412
CGGCTAA8100.0106.551571
GGCGCGG384050.099.306563
CGGGTAT347200.098.334581
CGCGGTG387250.098.316965
GCGCGGT383800.097.743734
CGGGTGT302300.097.385171
CGGAATA317900.097.081191
CGGATGC10700.095.680081
CGGGAGG1082300.094.862191
CGGGCGT441450.094.598641
CGGTAAT102650.093.820211
GGGAGGC472350.093.249212
CGGGTAA86350.092.712171
CGGGTTT587350.092.168511
GCGGTGG564250.091.5659566
Produced by FastQC (version 0.11.5)