FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14611046
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC714720.4891641570357112TruSeq Adapter, Index 5 (100% over 50bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC594170.406658085944018No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT302280.2068845721244051No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT256240.17537416554571109No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA201200.13770403570011344No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT180130.12328343911859561No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA170620.11677466486656737No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA168260.11515944854324597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC277050.0115.0831761
CGGTTAA997200.0113.629821
CGGGTGC77650.0113.295271
CGGGTAC104000.0108.128811
CGGCTAA7900.0107.815621
GGGCGCG318750.0103.739222
GGCGCGG325950.098.1439743
CGCGGTG326600.096.9103245
CGGAATA168100.094.8535161
GCGCGGT332800.094.40774
CGGGTGT276750.092.243821
CGGGTAT318300.091.823151
CGGGTTT833400.091.0661241
CGGAGTG94400.090.479451
CGGGCGT411900.090.146271
CGGATAC14800.089.343721
CGGATAA75650.089.205931
CGGTAAT91050.088.771321
GCGGTGG469100.088.131566
CGGTGGT572600.087.7762767
Produced by FastQC (version 0.11.5)