FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19137575
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC882610.46119218344016943No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT652160.34077462792438434No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA480670.2511655734856689No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT374840.1958659861555082No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA321230.16785303258119172No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC236870.12377221251908875TruSeq Adapter, Index 7 (100% over 50bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT233270.12189109644246984No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA218980.11442411068277981No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT214060.111853252044734No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT212760.11117396012817717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC418050.0116.0882341
CGGGTGC131600.0115.2956541
CGGTTAA931350.0111.910861
CGGGTAC162600.0111.508581
CGGCTAA8550.0109.30311
GGGCGCG477300.0105.482652
CGGGTAT447550.099.046411
GGCGCGG494900.098.738243
CGGGTGT379000.097.752841
CGCGGTG500600.097.495135
GCGCGGT494800.097.111024
CGGGCGT581050.095.3239061
CGGATGC13450.095.15141
CGGGAGG1388150.094.2433551
CGGAATA262500.093.879331
CGGGTAA111400.093.668961
GGGAGGC592300.093.3790052
CGGTAAT139350.093.078471
CGGGTTT753300.091.251031
CGGTGGT901500.090.642637
Produced by FastQC (version 0.11.5)