FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18198800
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC920770.5059509418203398TruSeq Adapter, Index 7 (100% over 50bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC808560.44429303030969075No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT318020.17474778556827922No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA255130.14019056201507793No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT208970.11482625228037013No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT194610.10693562212893157No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA191730.10535310020440908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC389650.0114.103771
CGGTTAA1074800.0113.109331
CGGGTGC109250.0112.8571241
CGGGTAC144450.0108.394821
GGGCGCG441850.0104.54852
CGGCTAA6950.098.51071
GGCGCGG457900.097.555863
CGCGGTG453950.096.8194665
GCGCGGT453450.096.034344
CGGGTAT461600.093.8806841
CGGGTTT1108750.092.855381
CGGGTGT388100.092.85331
CGGGCGT604600.090.936681
CGGTGGT784050.088.2006157
GCGGTGG648800.087.982476
CGGTAAT112400.087.871991
CGGATAC17500.086.07021
CGGAGTG100700.085.78441
CGGATGC11900.085.5499041
CGGAATA128750.085.3600851
Produced by FastQC (version 0.11.5)