FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13199989
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC573470.43444733173641276No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC384460.2912578184724245TruSeq Adapter, Index 7 (100% over 50bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT297790.2255986728473789No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT205820.1559243723612194No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT182250.1380682968750959No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA178230.1350228397917604No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA151230.11456827729174623No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA137440.1041212988889612No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT134180.1016515998611817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC260800.0115.495991
CGGTTAA998850.0114.38661
CGGGTGC76850.0113.3918841
CGGCTAA8150.0112.5497741
CGGGTAC98500.0109.512521
GGGCGCG300950.0104.181562
GGCGCGG311700.097.878153
CGCGGTG317800.096.373855
GCGCGGT311550.096.244814
CGGATGC8550.096.137871
CGGGTAT285200.094.504091
CGGGTGT253600.093.24441
GCTAATT8550.092.546523
CGGATAC11650.092.029721
CGGAAAT74000.091.7602541
CGGAATA148750.091.457781
CGGGTTT734700.091.28721
CGGGCGT376650.091.136461
CGGACGC12050.090.4576951
GCGGTGG445250.090.139876
Produced by FastQC (version 0.11.5)