FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12876376
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT1091960.8480336392786293No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA748550.5813359286805543No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA610780.47434153833345655No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT574890.4464687890443709No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC574390.4460804810297556No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT434730.33761828638741215No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA327590.2544116450156473No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA322920.2507848481591404No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA277350.2153944557071027No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA267420.20768265853684298No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAG225980.17549969028552753No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGAT205040.15923735063343908No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA203490.1580335957881317No Hit
CGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGAT189970.14753374707293418No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT181560.14100240626710497No Hit
CGGAATAGAATGGAATGTAATGGAATGGAACGGAATGGAATGGAATGGAA181220.14073835681716657No Hit
CGGTGGATTTTTCGGTTTAAGTTTTGGTAATACGGTGAAATTTCGTTTTA178960.13898320459110544No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT170250.13221887897650705No Hit
CGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA166630.12940752895069235No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT153100.11889991407520253No Hit
CGGAATTGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAATGGAA143830.11170068348423501No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA140260.10892816425988182No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT138460.10753025540726677No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT136610.10609351575319019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC262600.0116.174091
CGGGTGC82050.0115.425481
CGGTTAA653450.0112.1114351
CGGGTAC99200.0110.291971
CGGCTAA5850.0107.859921
GGGCGCG296600.0106.943432
CGGAATA342500.099.465681
GGCGCGG310700.098.9118353
CGGGTGT230350.098.5087361
CGGGTAT265850.098.453211
CGGATGC7650.098.043591
CGGGAGG900600.097.03621
GCGCGGT312150.096.8705754
CGCGGTG318400.096.501135
CGGGCGT339500.095.785891
GGGAGGC384100.095.5754552
CGGTAAT84950.093.2663041
CGGAAAT71800.093.103371
CGGATAC9100.092.2333151
CGGGTTT461150.091.945571
Produced by FastQC (version 0.11.5)