FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16873884
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC678930.4023554979991566No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT391790.23218720716581906No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT351520.20832192517146617No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA286720.16991938548350813No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA278450.165018320619011No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA240360.1424449759166295No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT231300.13707573194173908TruSeq Adapter, Index 14 (97% over 44bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA218210.12931818187205743No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT217740.1290396449329627No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG212900.12617130709207197No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT210600.12480825398586361No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA168910.10010143485637332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1214650.0114.157141
CGGGCGC327150.0112.8130951
CGGGTGC106350.0112.6914751
CGGGTAC117950.0109.432451
CGGCTAA7250.0103.470681
GGGCGCG380900.0101.762462
CGGAATA213800.099.6641161
GGCGCGG394200.095.382383
CGGGTGT313950.094.136071
CGCGGTG396650.093.758635
CGGGTAT355550.093.085231
GCGCGGT396350.092.899264
CGGATGC12150.092.1226351
CGGATAC14800.092.120981
CGGGTTT874100.091.610661
CGGAGTG99250.091.479571
CGGAAAT97600.091.135071
CGGGCGT461350.090.992371
CGGATAA89700.089.670111
CGGTAAT111950.089.6108551
Produced by FastQC (version 0.11.5)