FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005416509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12778463
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1342851.050869732924844TruSeq Adapter, Index 15 (97% over 40bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC592330.4635377509799105No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT217930.17054476739495195No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA175900.1376534877473136No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT142110.11121055795207922No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT139200.10893328876876664No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)