FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11434170
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC585620.5121666023856563No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT282300.24689155399998425No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA192950.16874858428727227No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT183630.16059757726183885TruSeq Adapter, Index 16 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT155700.13617079333261617No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA154560.13517378174366831No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT141470.12372563990215293No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT136510.11938776491866047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC280950.0115.147171
CGGGTGC87300.0114.288651
CGGGTAC109000.0112.848581
CGGTTAA564000.0111.887971
GGGCGCG316550.0105.835972
CGGCTAA3800.0105.025261
GGCGCGG328200.098.6514743
CGGGTAT305600.098.121091
CGCGGTG327800.097.591045
GCGCGGT324750.097.427364
CGGGTGT260850.096.38891
CGGGCGT394650.093.323221
CGGAATA138350.092.9556961
CGGATGC9500.092.171421
CGGGTTT542550.091.509961
GCGGTGG472650.091.163846
CGGTAAT80750.090.65921
CGGTGGT592250.090.400837
GGGAGGC296350.088.331632
CGGGAGG744800.087.710331
Produced by FastQC (version 0.11.5)