FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18159833
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC842490.4639304777747681No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT411100.2263787337691927No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT317620.17490248946672582No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA278370.1532888545836297No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT253530.13961031469837853TruSeq Adapter, Index 16 (97% over 40bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT248240.13669729231540842No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA243450.13405960286088534No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA212690.11712112110282072No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT199710.10997347828033441No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT196100.10798557453694645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1392250.0114.675741
CGGGCGC380850.0114.663521
CGGGTGC117650.0114.0580751
CGGGTAC136750.0110.9759451
CGGCTAA9500.0109.716321
GGGCGCG432000.0104.419562
GGCGCGG444150.098.549153
CGCGGTG450600.096.701975
GCGCGGT445000.096.568574
CGGAATA225050.095.592871
CGGGTGT358800.093.6731
CGGGTAT407450.093.363981
GCTAATT10350.092.5480653
CGGAGTG120600.092.0566941
CGGAAAT99650.091.925471
CGGGCGT534600.091.6577761
CGGATGC13400.091.5628361
CGGACGC15550.091.542821
CGGGTTT1017200.090.892091
CGGATAA105450.090.201781
Produced by FastQC (version 0.11.5)