FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12081081
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT688210.5696592879395478TruSeq Adapter, Index 18 (97% over 40bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC578200.47859955578478447No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT291930.2416422835009549No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA207030.1713671152440746No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT166610.13790984432601686No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA159490.13201633198221252No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT144640.11972438559099141No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT140490.1162892625254313No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA125010.10347583962064322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC276350.0115.3538061
CGGGTGC89550.0114.341521
CGGTTAA626050.0110.8383261
CGGGTAC112500.0108.150741
GGGCGCG316700.0105.132192
CGGCTAA4900.0100.779151
GGCGCGG326650.098.553673
CGCGGTG329050.097.310595
CGGGTAT293600.097.30941
CGGGTGT258150.096.982261
GCGCGGT328200.095.8420944
CGGGCGT376250.093.644141
CGGTAAT88800.093.19721
CGGATAC12650.092.654051
CGGAATA137000.092.327571
CGGTGGT593250.091.2228857
CGGAAAT73600.091.184281
CGGGTTT524700.090.746621
GCGGTGG485750.090.012316
GGGAGGC313400.089.983712
Produced by FastQC (version 0.11.5)