FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11683785
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC624440.5344500947252967No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT560410.479647648428998TruSeq Adapter, Index 18 (97% over 40bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT176420.15099558918620978No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT150130.12849431926383445No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT147390.1261491888116736No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA123900.10604440256303928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC295150.0115.907451
CGGGTGC88350.0114.2744451
CGGGTAC109050.0113.187041
CGGTTAA634450.0112.510461
GGGCGCG332850.0106.485942
CGGCTAA3900.0100.860621
GGCGCGG345950.099.399513
CGCGGTG345250.098.635565
CGGGTAT314350.098.305441
GCGCGGT342400.098.0657964
CGGGCGT410850.095.016781
CGGGTGT264150.094.98911
CGGATGC8600.094.9433061
CGGGTTT576650.092.047641
GCGGTGG490200.091.8740546
CGGAATA108350.091.6956941
CGGTAAT84050.091.686011
CGGTGGT608500.091.5722357
CGGATAC11500.090.694821
GGGAGGC283050.087.694772
Produced by FastQC (version 0.11.5)