FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14238340
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT688670.4836729562575412No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC665350.4672946424934367No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA563330.3956430314207977No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA456720.3207677299460471No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA412570.28975990178630373No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT394800.2772795143254059No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG350420.24611015048102516No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA340110.23886913783488806No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT270640.19007833778375852No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT268100.18829442196211074TruSeq Adapter, Index 18 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA213710.15009474419068514No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA210430.1477911048619432No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA196420.13795147468033492No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT187620.13177097892029546No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA175760.12344135622551505No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT163680.11495722113673364No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAG158070.1110171550897085No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGAT156610.10999175465679287No Hit
TGGTTAGTGGGGGTAGGTTTTTTAGGGAGAGGAGGGTGGATGGAATTAAG156570.10996366149424722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC102750.0114.373021
CGGGCGC312350.0113.214971
CGGTTAA710000.0112.322861
CGGGTAC116900.0110.9741061
CGGCTAA5850.0105.8902051
GGGCGCG358000.0103.707072
CGGATGC9250.099.808681
CGGGTGT273000.097.635721
CGGAATA272500.096.1755141
GGCGCGG375500.096.005883
CGGGTAT314800.095.740161
GCGCGGT372100.094.947674
CGCGGTG379650.094.595315
CGGGAGG960850.094.380011
CGGTAAT102300.094.148351
CGGGCGT411000.093.649071
GGGAGGC409050.092.87342
CGGATAC10600.091.030621
CGGGTTT518650.090.266481
GCGGTGG552150.088.878226
Produced by FastQC (version 0.11.5)