FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14347411
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC662060.46144910743826884No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT398430.2777016703571118No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA296260.20649021624877129No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA268140.18689086135470712No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT228840.15949915981357193No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT212560.1481521648749032No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT161780.11275901972836772No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT156100.10880011731733343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC308700.0115.277751
CGGGTGC96350.0113.815431
CGGTTAA788150.0113.744851
CGGGTAC114450.0113.364671
CGGCTAA6250.0107.754011
GGGCGCG353550.0104.579592
CGGAATA207700.097.589971
CGCGGTG370000.097.259775
GGCGCGG370900.097.1200563
GCGCGGT365900.096.478914
CGGATGC9700.096.4634861
CGGGTAT352450.096.334921
CGGGTGT297850.094.084931
CGGGCGT460150.092.5288241
CGGGTTT670350.092.044881
CGGATAC13750.091.889991
CGGTAAT104850.091.742421
GCGGTGG531700.090.633436
CGGTGGT661800.089.590967
GGGAGGC333250.089.3675162
Produced by FastQC (version 0.11.5)