FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005416524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15311303
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC654010.42714196172592234No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT326380.21316278568845512No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT290620.189807490583917No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA256270.1673730837930645No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA232310.1517245135832006No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT196850.12856515216242537No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT189020.12345128301621358No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA172530.11268146153204596No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT160240.10465471162055902TruSeq Adapter, Index 19 (97% over 40bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT157910.10313296001000045No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)