FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005416534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26274965
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC1050530.3998216553285609No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1017630.3873002304665296TruSeq Adapter, Index 4 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT734000.2793533692623377No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA586560.22323911754021367No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA503370.1915778003890776No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA497330.18927903424419404No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT401440.15278421874206113No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG300310.11429510943211532No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT284290.10819805088227519No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT273100.10393924406749923No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)