FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15872664
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC611080.38498893443469856TruSeq Adapter, Index 6 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT479100.30183969118227416No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC463840.2922256780588312No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA355600.22403296636279835No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT273630.1723907215575155No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA258360.16277040829441108No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT205280.1293292669711902No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA189890.11963335203214785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC80200.0114.25611
CGGGCGC243950.0112.662461
CGGCTAA6850.0110.525861
CGGTTAA511500.0110.142741
CGGGTAC91750.0107.797251
GGGCGCG281650.0102.7280652
CGGGTGT301300.095.246511
CGGGTAT350850.094.759581
GGGAGGC458300.094.19332
CGGGAGG1108700.093.315251
GGCGCGG300500.093.272743
CGGGTAA82850.093.050361
CGCGGTG297000.092.6724555
CGGGTTT620300.092.586821
CGGATGC7750.092.263341
GCGCGGT297800.092.100844
CGGAATA193700.091.5736851
CGGTAAT113350.090.648061
CGGGGAC24400.090.3774641
CGGGCGT449200.088.873821
Produced by FastQC (version 0.11.5)