FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15963840
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC649730.40700107242367756No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT501190.3139532844227955No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA324450.2032405736965542No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA263760.16522340489506285No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT261600.16387034698418426No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC232880.1458796880950949TruSeq Adapter, Index 6 (100% over 50bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA198690.12446253532984546No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT193430.1211675887505763No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC171410.1073739150480085TruSeq Adapter, Index 6 (100% over 50bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT160530.10055851223765712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC102250.0113.8315661
CGGGCGC326000.0113.684451
CGGTTAA597100.0109.190781
CGGGTAC124800.0107.86121
GGGCGCG370550.0105.049732
CGGCTAA5050.099.03011
CGGGTAT366500.097.759171
GGCGCGG384950.097.675793
GCGCGGT382300.096.953144
CGGGTGT323150.096.6503451
CGCGGTG388800.096.494495
CGGGCGT483450.093.949691
CGGGGAC29000.093.615151
GGGAGGC479150.092.755382
CGGGAGG1112900.092.3184361
CGGTAAT106200.092.2751161
CGGAATA205600.091.9390261
CGGGTTT583100.090.912031
CGGGTAA89350.090.8864441
GCGGTGG561800.090.534316
Produced by FastQC (version 0.11.5)