FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13139711
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT646740.4922026062825887TruSeq Adapter, Index 13 (97% over 40bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC510730.38869195829345105No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT283510.21576578054113976No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA248140.18884738028104273No Hit
AAGTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAA208860.15895326769363496TruSeq Adapter, Index 13 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA199660.1519515916293745No Hit
AAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAA179590.13667728308484106TruSeq Adapter, Index 13 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT176580.1343865173290341No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT144660.11009374559303473No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG132190.10060343031897732No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC257250.0112.938641
CGGGTGC75700.0111.511371
CGGTTAA547650.0111.040841
CGGGTAC97400.0109.542281
GGGCGCG308150.0101.3510742
CGGCTAA5200.096.232351
CGGGTAT260400.095.71851
CGCGGTG316950.095.570235
GCGCGGT314550.094.540624
GGCGCGG320050.094.161283
CGGGTGT231350.094.012921
CAGAAGA38400.092.019574
CGGGCGT362750.091.259511
GGGAGGC345800.089.937422
CGGGTTT485700.089.794031
CGGGAGG782800.088.8259051
CGGTGGT554250.088.557617
CGGAATA128000.088.3812561
GCGGTGG458050.087.831746
CGGAATG579400.085.544091
Produced by FastQC (version 0.11.5)