FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17916675
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC503000.28074405546788117No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA384740.2147385047727885No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG301890.1684966658155043No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT225060.12561482529543008No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA219140.12231064078574846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1048350.0112.476761
CGGGTGC109950.0110.9628141
CGGGCGC299750.0110.8081361
CGGGTAC119300.0107.803571
GGGCGCG355800.099.802152
GGCGCGG367200.093.189213
CGGGCGT462000.092.478511
CGGGTAT376450.092.35771
CGGGTGT335750.092.350781
CGGAATA168850.092.3462141
GCGCGGT363300.092.1266864
CGCGGTG369400.092.118195
CGGGTTT863450.088.5834051
CGGAATT213700.087.89241
CGGATAA83450.087.220581
CGGTAAT124200.087.066721
CGGTGGT684600.086.836387
CGGAAAT102450.085.8581161
CGGATAC15750.085.775671
GCGGTGG561950.085.075816
Produced by FastQC (version 0.11.5)