FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19149219
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC614920.3211201459443333No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA390800.20408143016172098No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG341820.17850336350532103No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT260070.13581232738525786No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA244370.12761355959216927No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT236140.1233157341821617No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC330800.0114.022331
CGGGTGC113050.0112.793331
CGGTTAA994250.0111.734491
CGGGTAC136850.0106.997211
CGGCTAA6350.0103.026321
GGGCGCG399600.0100.004762
CGGGTAT397500.095.4730451
CGGGTGT343650.094.996171
CGGGCGT468100.094.388891
CGCGGTG412400.092.9366765
GGCGCGG414800.092.785863
CGGAATA181800.091.763121
GCGCGGT415050.090.753644
CGGTAAT132950.090.4975361
CGGGTTT868650.089.9046551
CGGGAGG938600.089.7563551
CGGAATT224350.088.436021
CGGATGC13800.088.349481
CGGATAC17200.088.174081
GGGAGGC377300.086.522042
Produced by FastQC (version 0.11.5)