FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18626351
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC759410.40770733892000643No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA285120.15307346028215618No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT281500.15112997709535272No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT278340.1494334558604635No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT269600.14474117877409268TruSeq Adapter, Index 15 (97% over 40bp)
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG218640.11738208949246152No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA195930.10518968530121653No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT191430.10277375316292495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC381500.0114.933611
CGGGTGC120600.0113.84231
CGGTTAA1011400.0112.308311
CGGGTAC151600.0108.926971
CGGCTAA5900.0103.848531
GGGCGCG447700.0103.053972
GGCGCGG456400.097.635733
CGCGGTG459350.096.736925
CGGGTAT430300.095.968441
CGGGTGT365900.095.577621
GCGCGGT457500.095.47794
CGGGCGT545650.094.704551
CGGAATA177550.091.834011
CGGGTTT916650.091.5474551
CGGTAAT129800.091.383051
CGGATAC19150.091.015221
CGGATGC14750.090.338151
CGGTGGT829200.090.251987
GCGGTGG675650.089.4004066
CGGGTAA93650.089.0544051
Produced by FastQC (version 0.11.5)