FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005416611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19871113
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC680640.3425273662325809No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA317950.1600061355395644No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT287220.14454147585995814No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG274940.1383616509050097No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT263750.13273036090127413No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT236200.11886601419860077TruSeq Adapter, Index 18 (97% over 40bp)
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT219950.11068831423785873No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA215710.1085545636019482No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)