FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17576384
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC753350.42861489598770713No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC432320.24596640583182525TruSeq Adapter, Index 12 (100% over 50bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA299720.17052426710750063No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT287420.16352624066474652No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG247320.14071153657088967No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT237670.13522121501214357No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA191340.1088619820777698No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT186960.10637000192986225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC371850.0114.857161
CGGGTGC121600.0112.342851
CGGTTAA1014900.0112.177251
CGGGTAC145550.0110.5909041
GGGCGCG434050.0103.624282
CGGCTAA5700.099.250881
GGCGCGG447000.097.107763
CGGGTAT414400.096.281011
CGCGGTG450500.096.128845
GCGCGGT443400.096.085034
CGGGTGT363600.095.041721
CGGGCGT507450.093.9758761
CGGATGC14000.093.57941
CGGAATA166950.092.45581
CGGGTTT881200.091.434261
CGGTGGT806900.090.619137
CGGTAAT124950.089.790471
GCGGTGG656050.089.748596
CGGATAC15950.088.485731
CGGGTAA91050.088.034061
Produced by FastQC (version 0.11.5)