FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17451462
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC584060.3346768310872751No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA349840.2004645799876251No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG289240.165739695619771No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT268920.15409597201655656No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA197130.11295901741642048No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1090850.0113.113811
CGGGCGC322900.0112.863051
CGGGTGC112800.0111.7199251
CGGGTAC135200.0108.3050461
GGGCGCG383050.0100.202682
CGGGTAT395350.096.0631561
CGCGGTG395800.094.192785
GGCGCGG395550.093.879343
CGGGTGT350550.093.67511
CGGGCGT493150.093.360561
GCGCGGT392050.093.273194
CGGAATA174200.092.255061
CGGGTTT979350.089.9161151
CGGAAAT102550.089.873061
CGGTAAT123900.089.417421
CGGTGGT729050.088.1259467
CGGAATT220950.087.573011
CGGAGTG106750.087.5632861
GCGGTGG586950.087.2193156
CGGATAA87850.086.421671
Produced by FastQC (version 0.11.5)