FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13939609
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA691480.49605408587859245No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT558620.4007429476680443No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA451560.32394021955709085No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG395750.2839032285625802No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA378310.27139211723944334No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT370290.26563872774336783No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT333210.23903826857697374No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA318010.2281340889834141No Hit
TGGTTAGTGGGGGTAGGTTTTTTAGGGAGAGGAGGGTGGATGGAATTAAG253460.18182719472260664No Hit
TGGGTGTGGTGGTTTATGTTTGTAATTTTAGTATTTTGGGAGGTTGAGGT245320.1759877195981609No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA213420.15310329005641407No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAATGGAA192280.13793787185852915No Hit
TGGAATTGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAATGGAA185130.13280860316813764No Hit
TGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT183760.13182579224424446No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAA179660.12888453327492902No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA157450.11295151822407644No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAG144040.10333144925370576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA428950.0107.219431
CGGGTGC55600.0106.720381
GGGAGGC345600.095.9851152
CGGGAGG808200.095.0676351
CGGCTAA3950.093.5098651
CGGAATA97200.092.120341
CGGGTGT209750.091.8829961
CGGTAAT76350.091.371481
CGGGTTT439500.091.131191
CGGGTAC48100.090.291171
CGGGTAA62200.090.2242661
CGGGTAT200850.088.5391541
CGGGCGC99150.086.763551
CGGGAAG116800.085.893861
CGGGATA56800.085.166841
GCGGAGT212400.085.058687
CGGTATT189350.084.288871
CGGTATG53800.083.3826141
CGGATAA52850.081.8379061
GGAGGCG404600.081.3969043
Produced by FastQC (version 0.11.5)