FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17346256
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC483020.27845778362777535No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA338550.19517179960909142No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG334140.192629464248654No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT265600.1531166149052568No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA257110.14822218696645548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1095250.0113.458821
CGGGCGC277600.0113.062661
CGGGTGC90050.0112.3710561
CGGGTAC106400.0108.801551
GGGCGCG325850.0102.411262
CGGAATA174600.096.251631
GGCGCGG337800.095.5820853
GCGCGGT336700.093.896944
CGGGTAT338400.093.575031
CGGGTGT299800.093.39991
CGCGGTG343750.093.338235
CGGGCGT408650.093.036041
CGGAATT206950.090.0881041
CGGGTTT808700.089.6706161
CGGATAA83250.089.536621
CGGAAAT103650.089.247271
CGGTAAT118900.087.556841
CGGATGA64500.087.342191
CGGAGTG97000.086.718451
CGGTGGT632100.086.703167
Produced by FastQC (version 0.11.5)