FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18444674
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC595290.3227435735649218No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT295380.16014379001764956No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG293270.1589998283515339No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA274430.1487854976455534No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA265960.14419338612327873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1144650.0113.259311
CGGGTGC106450.0113.230461
CGGGCGC316000.0112.9811551
CGGGTAC127350.0108.288911
CGGCTAA8250.0102.4009251
GGGCGCG375250.0100.8801652
CGGGTGT311200.095.740171
CGGGCGT412700.095.648011
CGGGTAT347450.095.597121
CGGAATA174700.094.535741
GGCGCGG392300.093.2142943
CGGTAAT123700.093.159991
CGCGGTG390350.092.793195
GCGCGGT388300.092.088724
CGGATAC15500.091.735131
CGGGTTT805350.091.358551
CGGAATT222050.090.425831
CGGATAA98250.090.405891
CGGGAGG811350.090.1769941
CGGATGC12500.089.002271
Produced by FastQC (version 0.11.5)