FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005416700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21204401
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC738990.3485078404242591No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC363670.1715068489791341TruSeq Adapter, Index 4 (100% over 50bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT339330.16002809982701233No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA252760.11920166950247733No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG233400.11007148940448731No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)