FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12094185
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT483150.39948950673402134No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA360250.2978704228519739No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC332760.27514049107070876No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT258090.2134000761522996No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT200870.16608808282658152No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA198010.16372331000393991No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT155990.1289793400712822No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA148380.1226870599383092No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT143510.11866033139066419No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA141760.11721335501317369No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT136940.11322796864774269No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA128130.10594347614163335No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT123350.10199116352197359No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACCC152.700875E-4119.016214
CGGGTGC62250.0114.631641
CGGGCGC180700.0113.364571
CGGCTAA3900.0112.925421
CGGTTAA493750.0111.20671
CGGGTAC70850.0108.1933751
CGGCTAT750.0103.15891
GGGCGCG218300.0101.2713552
CGGGCTT1600.096.711481
CGGTCTA800.096.711481
CGGGTAT216700.095.657541
GGGAGGC304550.095.120372
CGGATGC7600.094.7537461
CGGGTGT196050.094.743961
GGCGCGG226300.094.7186053
CGCGGTG228700.093.7929845
CGGAATA167650.093.576441
GCGCGGT226700.093.317744
CGGGGAC15000.092.049491
CGGGAGG704500.091.903621
Produced by FastQC (version 0.11.5)