FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21892935
Sequences flagged as poor quality0
Sequence length125
%GC29

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT767060.3503687376772461No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA588890.2689863191024867No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA473230.2161564906669663No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT439860.20091413051744775No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC333030.15211756669446103No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT315280.14400992831705753No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA252100.11515130337709403No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA245660.11220971514326425No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA242020.11054707831544744No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA230820.10543127269139566No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT220860.1008818598328639No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC74300.0110.6966861
CGGTTAA568250.0105.6937261
CGGGCGC193600.0101.602521
CGGCTAA6250.0101.154451
CGGGTAC79350.099.818341
GGGAGGC541650.096.493692
CGGGAGG1241150.094.9462661
CGGGTGT280350.092.629011
GCGGAGT356900.090.16697
CGGAATA320000.090.0981451
CGGGTTT597950.088.703881
GGGCGCG239450.088.537312
CGGGTAA91500.088.323731
CGGGGAC21500.087.661281
CGGGTAT300200.087.656471
CGGGAAG176100.086.500921
CGGATGC10150.084.0291
CGGTAAT118250.083.777551
CGGATGA60000.083.102521
GGCGGAG642250.081.944356
Produced by FastQC (version 0.11.5)