FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17555862
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC617770.35188816134462664No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT589850.3359846414832835No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA477810.27216550232623155No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA448590.2555214890616023No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT345290.19668074401587346No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT303470.17285964084247188No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT246040.14014692072653567No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA236970.13498055521284003No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA230500.13129517650571645No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG226280.12889142099658793No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA204410.11643404351207591No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA199760.11378535556955278No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA181940.10363489984143187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC106400.0113.92911
CGGTTAA1127650.0113.707531
CGGGCGC309900.0112.525821
CGGCTAA8550.0110.720881
CGGGTAC120000.0108.459461
CGGAATA319400.0100.082231
GGGCGCG367350.099.925732
CGGATGC13100.096.3525161
CGGGTAT346400.095.9939351
CGGGTGT315700.095.0882341
CGGATGA86350.093.9792561
CGGGCGT442450.093.240561
CGGTAAT121600.093.126971
GGCGCGG383100.092.205663
CGCGGTG383250.091.8540345
CGGATAA101700.091.093451
GCGCGGT380300.090.926234
CGGGTTT760450.090.335491
CGGAATT266250.089.7607041
CGGGAGG831800.089.6586151
Produced by FastQC (version 0.11.5)