FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20046058
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC834170.41612670181838246No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA509250.25403997134997813No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC348020.17361019308634146TruSeq Adapter, Index 12 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT306140.15271830501538008No Hit
TGGATGTTAGAGGGGTGTTTTGGGTGATTTTTGGGATTTAGAAGTGAAAG299570.14944085266040835No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA237620.11853702109412236No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT237190.11832251508002221No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA231810.1156386956477927No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT218040.10876951468463276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC426950.0118.294531
CGGGTGC126950.0116.876151
CGGTTAA921250.0114.707971
CGGGTAC159750.0113.128291
CGGCTAA6200.0108.286581
GGGCGCG511400.0106.2404562
GGCGCGG529800.099.5648963
CGGGTAT436150.098.055041
CTGGTAC251.5213975E-597.654811
CGGGTGT376500.097.444071
CGGGCGT572200.096.820681
CGCGGTG545300.096.513245
GCGCGGT541850.095.334734
CGGTAAT140100.094.230611
CGGAATA200750.091.908621
CGGAAAT122750.091.787561
CGGTGGT961350.090.409397
CGGATAC19950.089.945221
CGGGAGG1103850.089.938231
GGGAGGC457700.089.935452
Produced by FastQC (version 0.11.5)