FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16598870
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC499260.3007795108944163No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT237330.1429796124675957TruSeq Adapter, Index 18 (97% over 40bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT216140.13021368322060478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA888600.0112.5106051
CGGGCGC274200.0112.245411
CGGGTGC92900.0110.838581
CGGGTAC111850.0107.8021241
GGGCGCG320800.0100.781042
CGGGTAT356450.093.587741
CGGGTGT315400.093.132011
CGCGGTG330400.092.7417455
CGGGCGT450850.092.542941
GGCGCGG339350.092.43333
CGGGTTT880400.091.8630141
GCGCGGT333200.091.605484
CGGAATA113100.088.307521
CGGAATT179700.088.019691
CGGATAA70650.087.144051
CGGTAAT100800.086.867411
CGGATAC13150.086.853041
CGGTGGT592050.085.933927
GCGGTGG485850.085.573126
CGGATGC13700.085.103041
Produced by FastQC (version 0.11.5)