FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15667578
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC588560.3756547438283058No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT522670.3335997433681198TruSeq Adapter, Index 16 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT450540.2875619958617726No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA321150.20497743812093994No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT260730.16641372393359077No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA254230.16226502909383952No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA237410.15152948337005248No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT182310.11636131634385352No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT178360.11384018640277394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC299500.0113.72231
CGGGTGC93200.0111.309721
CGGTTAA713000.0110.849871
CGGGTAC115250.0105.627661
CGGCTAA6150.0102.702491
GGGCGCG348950.0102.1672742
CGGGTAT343900.096.1116941
CGCGGTG355250.095.866965
GGCGCGG361900.095.108073
CGGGTGT288050.094.701781
GCGCGGT360250.093.793314
CGGGCGT421850.091.5307851
CGGAATA164450.090.440081
CGGGTTT679100.089.6654051
CGGTAAT89950.088.5024951
CGGTGGT626200.088.2341467
GCGGTGG514350.088.222536
CGGGAGG941600.088.190531
GGGAGGC384750.088.12612
CGGAATG902900.085.727411
Produced by FastQC (version 0.11.5)