FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005416772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23475537
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA1958640.8343323520139284No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG1267730.5400217255946052No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA955870.4071770541393792No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC694390.2957930206239798No Hit
TGGTGGGGATAGTGATGATTATGGTAGTGGAGGTGAAATATGTTTGTGTG466260.19861526490320539No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT453070.19299665008728023TruSeq Adapter, Index 16 (97% over 40bp)
TGGAAAAAAAGAATTATTTGGATATATAGGTATGGTTTGAGTTATGATAT408960.1742068775679125No Hit
TGGTTAGTGGGGGTAGGTTTTTTAGGGAGAGGAGGGTGGATGGAATTAAG408530.17402370816906126No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT364190.15513596132007546No Hit
TGGAGGAGGAGATTTTATTTTATATTAATATTTATTTTGATTTTTTGGTT329040.14016292790235216No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA293360.12496412755116101No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA277960.11840410722020969No Hit
TGGATAGGTTGAGAAAGTGTTGTGGGAAGAAAGTTAGATTTATGTTGATG273390.11645739988823259No Hit
TGGTAATGATGTTGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGT265320.11301977884467564No Hit
TGGTGGGGTTGAAGAAGGTGGTGTTGAGGTTGTGGTTTGTTAGTAGTATA256060.10907524713918153No Hit
TGGTTGTTAGGTGTTTAATGGGGTTTAGTAGGGTGGGGTTATTTTTGTTA247330.10535648236715522No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)