FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12553031
Sequences flagged as poor quality0
Sequence length125
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CNGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC463390.36914590587723395No Hit
CNGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT234860.18709425635928087No Hit
GNTCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC229920.18315895181012457TruSeq Adapter, Index 5 (98% over 50bp)
CNGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT137240.10932817739396963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGG273900.097.867523
CGCGGTG285900.094.0665055
GCGCGGT281650.093.368634
CGGTGGT493100.091.176447
CAGAAGA14600.088.097014
GCTAATT5250.087.974973
GCGGTGG408400.087.882666
GCAGAAG16100.077.710413
ACGGTGG133700.073.025816
GGTGCGG113750.071.801963
GGTACGG139650.070.123763
TAAGCGA91350.069.9524467
CGTAGTG162100.069.933645
AAGCGAT94700.068.2187358
GGCGTGG231300.067.76613
GGCGTAG169950.067.116213
GAAGATC35450.066.792876
GGAGGCG245150.065.821613
CGTGGTG234750.065.101555
GGCGGAG246250.065.029016
Produced by FastQC (version 0.11.5)