FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12264882
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT549280.4478477656776478No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA420670.34298740093871266No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC417220.34017449169099223No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT309110.25202851523561337No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT268770.21913786043762998No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA231930.18910088168805864No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA213590.17414761919437954No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT202140.164812021836003No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT179030.14596960655634517No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT172820.1409063699104484No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT159110.1297281131608115No Hit
CGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA129870.10588768811636345No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA129830.10585507467581017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC215500.0114.2361451
CGGGTGC66600.0113.996331
CGGTTAA533650.0110.8554841
CGGCTAA6450.0109.022961
CGGGTAC85100.0107.141461
GGGCGCG250300.0102.393832
CGGGTAT253600.096.627231
GGCGCGG261400.095.373563
CGCGGTG260050.095.204985
CGGGTGT221950.095.048181
CGGAATA200950.094.927781
GGGAGGC371950.094.744232
GCGCGGT260500.094.035284
CGGGTTT484650.093.302821
CGGGAGG891850.092.932691
CGGTAAT75500.092.428431
CGGGTAA64250.090.71131
CGGGCGT350100.090.4023741
CGGATGC7500.088.1977161
CGGAATG1081650.087.9034041
Produced by FastQC (version 0.11.5)