FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005416798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27697084
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC974900.3519865123707608No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT680800.24580204905325054No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT399060.14408014937601374No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT390780.14109066499563636No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT340140.12280715182869069TruSeq Adapter, Index 18 (97% over 40bp)
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA326020.11770914223316793No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA311010.11228979917163842No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA295670.10675130999349966No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)