FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12209075
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC362320.2967628587751324No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT292240.2393629329003221No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT266040.21790348572680568No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA234200.1918245239708987No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA172470.1412637730540602No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT165480.13553852359822507No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT161170.13200836263189472No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA134240.10995099956384903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC180800.0114.511721
CGGTTAA914750.0114.274821
CGGGTGC58400.0110.850021
CGGCTAA5600.0109.740781
CGGGTAC71650.0108.171251
GGGCGCG223750.099.977682
CGGAATA154450.095.84241
CGGGCAT1750.095.463831
GGCGCGG231700.093.691013
CGCGGTG231850.092.704635
CGGGTAT208150.092.356621
GCGCGGT232650.091.4120254
CGGGCGT277500.090.282121
CGGAAAT67550.090.27021
CGGGTTT592200.090.132081
CGGGTGT185300.089.996431
CGGATAC10650.088.516931
CGGTAAT73100.088.232211
CGGTGGT403800.087.380047
CGGGTAA53550.086.3497541