FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20273870
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT635730.3135711139511105No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC562790.2775937697144157No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA434810.2144681799774784No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT341450.1684187577408753No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA306830.15134259024054117No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT265000.1307101209586527No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT244990.12084027371192575No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT222750.10987048846618824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC115800.0113.6484151
CGGGCGC311500.0111.897971
CGGTTAA717550.0110.610031
CGGGTAC120550.0109.417581
CGGCTAA5900.0108.0947651
GGGCGCG370200.0100.422222
GGGAGGC654350.097.2566452
CGGGAGG1547450.097.171551
CGGGTAT416950.096.620971
CGGGTGT371800.096.6034551
CGGGTAA117250.094.145821
CGGAATA256300.093.0682141
CGGTAAT150900.092.8616261
GGCGCGG387450.092.696573
GCGCGGT383250.092.0517354
CGGGTTT754550.091.931261
CGCGGTG388900.091.3720865
CGGGAAG209400.089.974771
CGGGCGT512900.089.945861
CGGATGC10650.088.985741
Produced by FastQC (version 0.11.5)