FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17954962
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT596270.3320920423000617No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC535900.29846902488571125No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA431960.2405797350058441No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA373420.2079759344519916No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT344880.19208060702105634No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT233140.1298471141292307No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT201440.11219182752934816No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA198590.11060452258267102No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT197950.11024807515604877TruSeq Adapter, Index 16 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA190850.10629373651695836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC109300.0113.538321
CGGCTAA6500.0112.844991
CGGTTAA654600.0109.738091
CGGGCGC295950.0109.595131
CGGGTAC114000.0107.392681
GGGCGCG355500.097.3074342
CGGGTGT365750.095.609311
GGGAGGC542100.094.736692
CGGGTAT413450.094.487671
CGGTAAT130200.092.61061
CGGGAGG1338150.092.118581
CGGGGAC32150.092.000731
CGGGTAA106550.091.898881
CGGAATA267600.091.611911
CGGGTTT735200.091.2104341
CGCGGTG366900.089.436025
GGCGCGG375000.089.265073
GCGCGGT368400.089.104164
CGGAGTG117850.088.197931
CGGGATA99450.087.606441
Produced by FastQC (version 0.11.5)