FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20714954
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT402120.19412063381844827No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC341100.1646636531271081No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA319530.15425088561625577No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT317430.15323712521881536No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA313240.15121443185439853No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT257450.12428219729573138No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA228100.11011368888388554No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA214540.10356769317469883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA7950.0110.1768341
CGGGTGC82500.0108.048251
CGGTTAA627800.0106.784931
CGGGTAC87050.0102.53761
CGGGCGC209250.0101.430491
GGGAGGC639050.099.415752
CGGGAGG1450350.097.2815861
CGGAATA244300.094.0976641
CGGTAAT141950.093.649241
CGGGTGT333550.092.44631
CGGGTAA111750.092.297441
CGGGTTT707950.091.741111
CGGGAAG206550.090.322921
GCGGAGT403350.089.980597
CGGGTAT346300.089.0426251
GGGCGCG272750.088.1503752
CGGTATT320050.087.3534851
CGGGATA102000.086.340471
GGAGGCG742050.085.2417763
GGCGGAG728450.084.954956
Produced by FastQC (version 0.11.5)