FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20556851
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT1206810.5870597592987369No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA846570.4118189113692559No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA729760.35499600595441394No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT685300.33336817978590205No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT603370.2935128536953447No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC461490.2244945006411731No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA443050.21552425514977952No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA427210.20781879481443924No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT415100.20192781472220625No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAATGGAA366180.17813039555523363No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAA358490.17438955022829128No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA300410.14613619566537697No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAG293770.14290612895914845No Hit
TGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGAT280600.1364995056879091No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT270070.1313771258058931TruSeq Adapter, Index 18 (97% over 40bp)
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGAT266630.12970371775326872No Hit
TGGAATAGAATGGAATGTAATGGAATGGAATGGAATGGAATGGAATGGAA239780.11664237873787187No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA225080.10949147804787805No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT220200.1071175736011318No Hit
TGGAATTGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAATGGAA209520.10192222534472814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC91750.0111.532841
CGGTTAA647100.0109.010461
CGGGCGC255300.0107.2845461
CGGGTAC97100.0104.036481
CGGCTAA7650.0102.897311
GGGAGGC585950.096.428292
CGGGAGG1348150.095.48731
CGGGTGT316650.092.882751
GGGCGCG316200.091.659672
CGGGTAT343850.090.720871
CGGTAAT132000.090.263691
CGGAATA222500.089.597911
CGGGAAG197600.088.062261
CGGATGC10050.087.818731
CGGGTTT625750.087.732721
CGGGTAA102050.085.958971
GCGGAGT367700.085.600647
CGGGCGT437200.085.113011
CGGGATA94150.084.684241
CGCGGTG327000.084.539135
Produced by FastQC (version 0.11.5)